Expression of TGFbeta1 and its type I receptors ALK1 and ALK5 mRNA in brain arteriovenous malformation / 南方医科大学学报

<p><b>OBJECTIVE</b>To explore the expression of transforming growth factor beta1 (TGFbeta1) and its type I receptors activin-like kinase 1 (ALK1) and ALK5 mRNA in the development of brain arteriovenous malformation (BAVM).</p><p><b>METHODS</b>The mRNA expressions of TGFbeta1, ALK1and ALK5 were detected with semiquantitative RT-PCR in patients with BAVM.</p><p><b>RESULTS</b>The expressions of TGFbeta1 and ALK5 mRNA increased significantly in BAVM, and their relative expression quantity were 0.777-/+0.047 and 0.585-/+0.074, respectively. However, ALK1 mRNA expression declined significantlies with a relative expression of 0.173-/+0.044 in comparison with the control group (0.720-/+0.098, P<0.01).</p><p><b>CONCLUSION</b>The balance of TGFbeta1 and its type I receptors ALK1 and ALK5 mRNA expressions may play important role in the development of BAVM.</p>

The correlation between blood flow velocity in intracranial aneurysm cavity and the size of aneurysm / 中国脑血管病杂志

Objective: To explore the correlation of the size of intracranial aneurysms with the blood flow velocity in the aneurysmal cavity and the parent artery. Methods: The video data of serial digital subtraction angiography (DSA) images of 15 intracranial aneurysms in 15 patients were copied to a personal computer, then the pixel density values in the selected areas of images were measured with the changes of time by an image processing software, and the time-density curves were generated. The inflow coefficient Ka values of the curve were calculated by curve equation and the statistical analysis was performed so as to find out the blood flow velocity in various aneurismal cavities. Results: The Ka value that marked the blood flow velocity in aneurismal cavity (ACKa) in the central area of an aneurism had a negative correlation with the size of the aneurysm, r = -0.521, P = 0.046 (both sides). The size of the aneurysm had no correlation with the Ka value of the marked blood flow velocity in a parent artery (PKa), r = 0.107, P = 0.704 (both sides). Conclusion: The larger the aneurismal cavity, the slower the blood flow velocity in the central area of the aneurysm. The smaller the aneurysmal cavity, the fewer attenuation of blood flow velocity in the aneurysms, and the faster the blood flow velocity in the central area of the aneurysm. However, the flow velocity in parent artery is not associated with the size of aneurysm. The results of the study can be used to evaluate the natural outcome of aneurysm, the efficacy of embolization, and may partly explain the causes of aneurismal recurrence.

Mechanism of Ca2+ on the hyperthermia-induced apoptosis of rat hippocampal neurons in vitro / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the mechanism of Ca(2+) on the apoptosis induced by hyperthermia in neonate rat hippocampal neurons to provide the applicative evidence of dantrolene for preventing brain injuries.</p><p><b>METHODS</b>Dantrolene, Ca(2+) specific blocking agent, was used in the hyperthermia-induced apoptosis of primary hippocampal neurons in vitro to observe its effect on the apoptosis, fluorescent intensity, and dynamic change of Ca(2+) by flowcytometry and laser confocal microscopy.</p><p><b>RESULTS</b>The rate of apoptosis was decreased significantly after hyperthermia treatment by dantrolene sodium. The intracellular Ca(2+) fluorescent intensity in 42 degrees C treatment group (107.35 +/- 6.0) was significantly lower than that in control group (159.12 +/- 33.8). The concentration of Ca(2+) began to decrease 20 approximately 25 s after adding dantrolene sodium, and reached the lowest level about 50 s later, and then kept lower than the basal level.</p><p><b>CONCLUSION</b>Dantrolene sodium has an important protective effect on hippocampal neurons apoptosis induced by hyperthermia and may have some applicative value of preventing heat-induced brain injury.</p>

Relationship of bleeding complications and impairment of draining veins after ?-n-butyl cyanoacrylate embolization of brain arteriovenous malformations / 中华放射学杂志

Objective To investigate the causes,consequences and management of injuries to the draining veins after embolization of brain arteriovenous malformations(BAVMs)with ?-n-butyl cyanoacrylate (NBCA).Methods The angiographic imaging data of 189 BAVMs patients who underwent NBCA embolization were studied retrospectively.The status of the draining veins before and after NBCA embolization was observed and compared.The intracerebral hemorrhage(ICH)complications and their relation to their angiographic features were analyzed.Results Twenty-three patients out of 189 patients showed injuries to the draining venous system,including 10 low-grade injury,6 moderate injury,and 7 high- grade injury.Six patients suffered from ICH after embolization,of whom 4 patients were due to injuries of the draining veins(2 moderate and 2 high-grade).In the 3 months follow-up evaluation of 4 patients with ICH, one died,one was in vegetative state,and the other two patients suffered from residual severe or minor (1 patient for each)permanent neurological deficits.Conclusion Our findings suggest that injury of the draining veins is the major cause of ICH and may lead to serious consequences after embolization of BAVMs with NBCA.